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Funding Agency:  Cancer Prevention Research Institute of Texas Grant Number:  RP170638 Quick Links Department of Radiology Nuclear Medicine Residency Radiology Research Program

Project 2: Wellstone Center - UT Southwestern, Dallas, TX Wellstone Center Micro Navigation About Us Award Announcements Cores Research Project 1 Project 2 Project 2 Translating “myoediting” to young men with DMD is the fundamental goal of the UT Southwestern Wellstone MDCRC.

1 FEG_Registers 1 For Krios ONLY: LowDose -> FEG_Registers -> select “300kV_Dan_…” -> click “Set” Note: the file navigator window on the right shows the location of the FEG_Registers configuration

Project 1  seeks to correct muscular dystrophy in a mouse model of Duchenne muscular dystrophy using a gene-editing technique developed at UT Southwestern called Myoediting.

Core provides expert training in the following procedures with mice: Transcardial Whole Body Perfusion All Organs Survey Dissection Organ Specific Dissection Organ Specific Grossing Embryo Collection and Staging (E8 - E15) The Core maintains animal use protocols covering the euthanasia and necropsy of

Department of Health and Human Services, Office of Human Research Protection. All human subject research conducted by UT Southwestern faculty, staff, or students on behalf of UT Southwestern is overseen by the Human Research Protection Program (HRPP) Department.

1 FRDA fibroblasts Short alleles <499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele

Chen Lab Journal Club The Last Friday of every month, 4:20PM at NA6 small conference room. Date 09.15. Mingjian 10.15. Haocheng 11.15. Seoyun 01.16. Peiqing 02.16. Hui Y. 03.16. Conggang 04.16. Youtong 05.16. Daxing 06.16. Philip 07.16. Tuo 08.16. Hua

1  9 – FEG_Registers  1    FEG_Registers ‐> select “4350_UT_0314…” ‐> click “Set”      Note: the file navigator window on the right shows the location of the FEG_Registers configuration  files. 

Resources: Structural Biology Lab - UT Southwestern, Dallas, Texas Resources Vitrobot Training Materials Vitrobot Protocol Sample preparation slides Training resources on Vitrobot operation and grid preparation EM Operation Protocols Protocol of using Multi Records in SerialEM Protocol of using Hole